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Nucleic Acids

Easychemistry

In the Watson–Crick double helical model of DNA, which of the following base-pair combinations and number of hydrogen bonds is CORRECT?

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About This Question

Subject
chemistry
Chapter
biomolecules
Topic
nucleic acids
Difficulty
Easy
Year
2025
Tags
Nucleic AcidsDNABase PairingHydrogen BondsWatson Crick

Solution

Correct Answer:

A–T: 2 hydrogen bonds; G–C: 3 hydrogen bonds

According to Watson and Crick's model of B-DNA, complementary base pairing occurs between Adenine (A) and Thymine (T) via 2 hydrogen bonds, and between Guanine (G) and Cytosine (C) via 3 hydrogen bonds. Because G–C pairs have one additional hydrogen bond, DNA rich in G–C content has a higher melting temperature (Tm). This stronger G–C interaction is exploited in PCR primer design — primers with higher G–C content bind more stably. The two strands run antiparallel (one 5'→3' and the other 3'→5').

This easy difficulty chemistry question is from the chapter biomolecules, covering the topic of nucleic acids. It appeared in the 2025 exam.

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