Numerical: Pcr Cycles
A PCR starts with 500 template molecules. After 5 perfect cycles, theoretical double-stranded product count is:
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About This Question
- Subject
- biology
- Chapter
- biotechnology and its applications
- Topic
- numerical: pcr cycles
- Difficulty
- Medium
- Year
- 2025
Solution
Correct Answer:
16000
- Growth Pattern: PCR results in exponential amplification of the target DNA sequence.
- Formula:
- Final count=Initial count×2n
- where n is the number of cycles.
- Calculation:
- Given n=5.
- Final count=500×25=500×32=16,000.
- Manual Progression:
- 500 (Start) → 1000 (C1) → 2000 (C2) → 4000 (C3) → 8000 (C4) → 16,000 (C5).
This medium difficulty biology question is from the chapter biotechnology and its applications, covering the topic of numerical: pcr cycles. It appeared in the 2025 exam.
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