Numerical: Pcr Cycles

Question

A PCR starts with 500 template molecules. After 5 perfect cycles, theoretical double-stranded product count is:

RankGuru · Accepted Answer

1. **Growth Pattern**: PCR results in exponential amplification of the target DNA sequence.
2. **Formula**: 
   - $Final \ count = Initial \ count 	imes 2^n$
   - where $n$ is the number of cycles.
3. **Calculation**: 
   - $Given \ n = 5$.
   - $Final \ count = 500 	imes 2^5 = 500 	imes 32 = 16,000$.
4. **Manual Progression**:
   - 500 (Start) $	o$ 1000 (C1) $	o$ 2000 (C2) $	o$ 4000 (C3) $	o$ 8000 (C4) $	o$ **16,000 (C5)**.

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Numerical: Pcr Cycles

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