crispr-cas9

Question

Cas9 nuclease creates which type of DNA break guided by sgRNA?

RankGuru · Accepted Answer

1. **Components**: CRISPR-Cas9 uses a **single guide RNA (sgRNA)** to find a target DNA sequence and a **Cas9 protein** (nuclease) to cut it.
2. **The Cut**: Cas9 acts like a pair of molecular scissors that creates a **double-strand break (DSB)** at a very precise genomic location.
3. **Repair**: Once the DNA is broken, the cell's natural repair mechanisms (NHEJ or HDR) kick in to fix the break, which often results in gene silencing or precise gene editing.
4. **Precision**: This technology is revolutionizing biotechnology due to its ease of programming compared to older methods.

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crispr-cas9

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