Crispr-cas9
Cas9 nuclease creates which type of DNA break guided by sgRNA?
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About This Question
- Subject
- biology
- Chapter
- biotechnology and its applications
- Topic
- crispr-cas9
- Difficulty
- Hard
- Year
- 2025
Solution
Correct Answer:
Double-strand break
- Components: CRISPR-Cas9 uses a single guide RNA (sgRNA) to find a target DNA sequence and a Cas9 protein (nuclease) to cut it.
- The Cut: Cas9 acts like a pair of molecular scissors that creates a double-strand break (DSB) at a very precise genomic location.
- Repair: Once the DNA is broken, the cell's natural repair mechanisms (NHEJ or HDR) kick in to fix the break, which often results in gene silencing or precise gene editing.
- Precision: This technology is revolutionizing biotechnology due to its ease of programming compared to older methods.
This hard difficulty biology question is from the chapter biotechnology and its applications, covering the topic of crispr-cas9. It appeared in the 2025 exam.
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