Pcr Technique

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DNA polymerase used in PCR is heat-stable because it is sourced from:

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1. **PCR Cycle**: The Polymerase Chain Reaction requires a high-temperature step ($94 \u2013 96 \u00b0C$) for DNA denaturation.
2. **Requirement**: Standard DNA polymerases (like those from $E. coli$) would denature and lose activity at these temperatures.
3. **Taq Polymerase**: A thermostable DNA polymerase called **Taq polymerase** is used. 
4. **Source**: It is isolated from the bacterium **$Thermus \ aquaticus$**, which thrives in extreme heat conditions like hot springs. This allows the enzyme to remain active throughout the multiple high-heat cycles of PCR.

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Pcr Technique

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