Pcr Technique
DNA polymerase used in PCR is heat-stable because it is sourced from:
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About This Question
- Subject
- biology
- Chapter
- biotechnology and its applications
- Topic
- pcr technique
- Difficulty
- Easy
- Year
- 2025
Solution
Correct Answer:
Thermus aquaticus
- PCR Cycle: The Polymerase Chain Reaction requires a high-temperature step (942˘013960˘0b0C) for DNA denaturation.
- Requirement: Standard DNA polymerases (like those from E.coli) would denature and lose activity at these temperatures.
- Taq Polymerase: A thermostable DNA polymerase called Taq polymerase is used.
- Source: It is isolated from the bacterium Thermus aquaticus, which thrives in extreme heat conditions like hot springs. This allows the enzyme to remain active throughout the multiple high-heat cycles of PCR.
This easy difficulty biology question is from the chapter biotechnology and its applications, covering the topic of pcr technique. It appeared in the 2025 exam.
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