gene cloning

Question

Selectable marker in plasmid vectors commonly confers:

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1. **The Problem**: After a transformation experiment, most host cells do not take up the foreign DNA.
2. **Purpose of Marker**: A **selectable marker** is used to distinguish and selectively grow transformants from non-transformants.
3. **Common Examples**: Genes encoding resistance to antibiotics like **ampicillin**, **chloramphenicol**, **tetracycline**, or **kanamycin** are the most common markers.
4. **Mechanism**: When grown on a medium containing the antibiotic, only the cells that have successfully taken up the plasmid (carrying the resistance gene) will survive.

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gene cloning

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