Gene Cloning
Selectable marker in plasmid vectors commonly confers:
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About This Question
- Subject
- biology
- Chapter
- biotechnology and its applications
- Topic
- gene cloning
- Difficulty
- Medium
- Year
- 2025
Solution
Correct Answer:
Antibiotic resistance
- The Problem: After a transformation experiment, most host cells do not take up the foreign DNA.
- Purpose of Marker: A selectable marker is used to distinguish and selectively grow transformants from non-transformants.
- Common Examples: Genes encoding resistance to antibiotics like ampicillin, chloramphenicol, tetracycline, or kanamycin are the most common markers.
- Mechanism: When grown on a medium containing the antibiotic, only the cells that have successfully taken up the plasmid (carrying the resistance gene) will survive.
This medium difficulty biology question is from the chapter biotechnology and its applications, covering the topic of gene cloning. It appeared in the 2025 exam.
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