Chromatography

Question

In thin-layer chromatography (TLC), the Rf value of a compound is defined as:

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1. **Definition**: $R_f$ stands for **Retardation Factor**.
2. **Measurement**: It is a dimensionless ratio calculated from the chromatogram.
3. **Formula**: **$R_f = \frac{	ext{Distance traveled by the substance from baseline}}{	ext{Distance traveled by the solvent front from baseline}}$**.
4. **Range**: Since the solute can never move faster than the solvent, the $R_f$ value is always less than $1$ ($0 \le R_f \le 1$).
5. **Utility**: $R_f$ values are characteristic for a compound with a specific stationary phase and solvent system, allowing for the identification of components in a mixture.

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Chromatography

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